qPCR SYBR Green I Detection

qPCR SYBR Green I Detection

 
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Gene Expression Analysis

PerfeCTa SYBR Green FastMix products are the optimal choice for routine quantitative PCR applications such as transcript profiling, chromatin immune-precipitation (ChIP), and copy-number assays. FastMixes are formulated for optimal PCR efficiency so relative and absolute quantification results can be accurate. You can choose from fast or standard cycling conditions, and two or three stage protocols. Optical Clarity Technology ensures reproducibility and formulations are available for all real-time platforms. Hot-start technology maximizes specificity while enabling bench-top reaction assembly.

microRNA Profiling

PerfeCTa SYBR Green SuperMix products are recommended for microRNA quantification and other applications where PCR specificity is a challenge.
In the trade-off between PCR efficiency and specificity the SYBR Green SuperMix provides a more stringent option enabling specific amplification of short sequences such as small RNAs.
Standard cycling times are recommended for amplicons up to 250 bases. Optical Clarity Technology ensures reproducibility and formulations are available for all real-time platforms. Hot-start technology maximizes specificity while enabling bench-top reaction assembly.

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