Recovery of DNA fragment from agarose gel
Gel purification of DNA is a common technique used for the isolation of specific DNA fragments from reaction mixtures. However, most methods either fail to completely remove agarose (which can lead to problems in downstream manipulations), shear the DNA, or results in very low yields. The E.Z.N.A.® Gel Extraction kit uses HiBind DNA spin-column technology to recover DNA bands 70bp-20 kb in length from all grades of agarose gel with yields up to 85%. The DNA band of interest is excised from the gel, dissolved in Binding Buffer, and applied to a DNA HiBind spin column. Following three wash steps, DNA is eluted with deionized water or Elution Buffer and is ready for downstream applications such as ligations, PCR amplification, restriction enzyme digestion and various labeling reactions. This kit can be also used to purify DNA fragments from PCR products and enzymatic reactions. Two spin column formats (V-Spin column and Q-Spin Column) are are available for this kit.
- Speed - DNA recovery from agarose gel in 15 min
- Reliability - Optimized buffers guarantee pure DNA
- Quality - Purified DNA suitable for most applications